Nahla Elhesaisy

The British University in Egypt, Egypt

3D Nanofibers Scaffolds for Boosting the Repurposed Dermatological Effect of spironolactone

---

Biography

Nahla A.Elhesaisy is a teaching assistant, Department of Pharmaceutics and Pharmaceutical Technology, Faculty of Pharmacy, The British University in Egypt, Cairo, Egypt. She is also a M.Sc. student at Faculty of Pharmacy, Cairo university, Egypt. Nahla graduated from faculty of pharmacy, the British university in Egypt with cumulative degree: Excellent with high honours. She ranked 3rd among her class. She is an editorial board member for Open Journal of Pharmaceutical Science and Research. Also she is an editorial board member at MAT Journals "Trends in Pharmaceuticals and Nanotechnology". She is a member of the Egyptian National Nanotechnology Network, ENNN. She participated with many posters and oral presentation in many prestigious conferences and awarded best poster award in more than one conference. She was a Co-PI an awarded Young Investigators Research Grant. The British University in Egypt and also was a member in another granted project. She worked also as supervisor for fifth year graduation project for pharmacy students.

Abstract

Spironolactone (SP) proved its oral efficacy in the treatment of many dermatological diseases orally but its use has been accompanied with severe systemic side effects like cramps, gynecomastia, hypotension, irregular menstruation, hyperkalemia, and reduced libido. These challenges for the repurposed use of SP can be overcome via its reformulation in suitable nano-drug delivery system which proved a crucial role in such drug repurposing related obstacles. One of the most promising drug delivery systems that can be tailored for a wide range of drug release patterns are nanofibers (NFs). NFs are solid fibers with a diameter size starting from a few nanometers to 1000 nm with multiple unique functionalities and characteristics. NFs can be used in multiple routes of administration but topical route was the route of choice in our study. Polyvinylpyrrolidone (PVP) and Polycaprolactone (PCL) polymers were used for the fabrication of SP-PCL NFs and SP-PVP NFs via electrospinning method. the scanning electron microscopy showed that SP-PVP NFs have smooth homogenous surface with diameter size about 426.6 nm while SP-PCL NFs showed beadles fibers with fiber diameter about 500 nm. The entrapment efficiency (EE%) for SP-PVP NFs is of 96.34%± 1.2 and its loading capacity (DL%) of 11.89% ± 0.15. Solid state, and mechanical properties of NFs scaffolds were evaluated. The obtained values showed the durability of the prepared scaffolds. The in-vitro release study showed that higher amount of SP was released from SP-PVP NFs within 24 hours. While SP-PCL NFs provided more controlled pattern till 168 hours. Higher percentage of SP was retained in skin from NFs compared to pure SP gel. The pharmacological effect of both scaffolds was evaluated in two different in-vivo models and a significant enhancement for SP dermatological effects was achieved. Electrospun polymeric nanofibers are safe promising carrier of SP.