Manohar M

NGSM Institute of Pharmaceutical Sciences, India

Title: Fabrication and Evaluation of Quercetin and β-Carotene Loaded Nanoemulsions for Improved Oral bioavailability and Therapeutic Efficacy in Diabetes Mellitus

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Biography

Manohar M is presently working as an Assistant Professor in the Department of Pharmaceutics of NGSM Institute of Pharmaceutical Sciences, Nitte (Deemed to be University), Mangalore, India. He completed his Masters in Pharmaceutics and pursued his PhD in the development of Nano drug delivery systems at JSS College of Pharmacy, JSS AHER, Mysuru, India. His research interest lies in designing and developing nano delivery systems. He is currently working on the nanoemulsion based delivery systems of bioactive molecules of Flavonoids and Carotenoids for improved anti-diabetic properties. In the last five years of the research journey, he has conscientiously trained and contributed to optimization techniques, evaluation and characterization of various delivery systems, including drug release, pharmacokinetics and in-vivo anti-diabetic activities. He is passionate and believes that interdisciplinary research is the key to develop a viable product.

Abstract

The current research was intended to explore the novel combination of Quercetin and ?-carotene in a nanoemulsion based delivery system to improve their bioavailability and therapeutic effectiveness in Diabetes mellitus (DM).

The Quercetin nanoemulsion (Que-NE) and ?eta Carotene nanoemulsion (?C-NE) were prepared using the ultrasonication technique. The box-Behnken design (BBD) was employed to optimize and study the influence of independent variables such as % S_mix (5 to 7%), % amplitude (20-30%) and sonication time (2.5 -7.5 mins) on droplet size, Poly Dispersibility Index and % Entrapment efficiency. The results predicted that 9% Smix at 25% amplitude for 2.5 mins would produce Que-NE with a droplet size of 125.51 nm, 0.215 PDI, and 87.04 % EE Similarly, 7% S_mix at 25% amplitude for 5 mins would produce ?C-NE with a droplet size of 152.96 nm, 0.197 PDI and 73.32 % EE. The optimized Que-NE and ?C-NE exhibited appreciable stability concerning droplet size and PDI when stored at 4, 30,45 and 55°C for 45 days. Additionally, the Que-NE and ?C-NE, in contrast with their free drug, exhibited superior release and enhanced bioavailability in in-vitro and in-vivo studies. The STZ induced anti-diabetic study in rats revealed that the Que-NE and ?C-NE in combination had remarkable protective and therapeutic properties in managing body weight, blood glucose level, lipid profile, tissue injury markers alongside the structure of pancreatic ?-cells and hepatocytes were protected.

Thus, the study suggests that ultrasonically assisted Que-NE and ?C-NE, in combination, have great potential use as a substitute strategy in DM.