Mengmeng Yang

Southeast University, China

Title: Preparation of high Mg-loaded amorphous calcium phosphate with osteogenic and angiogenic properties

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Biography

Mengmeng Yang is from School of Materials Science and Engineering, Southeast University, Nanjing, 211189, Jiangsu, China. His focus is on the preparation and performance evaluation of bone repair materials (mainly about preparation and modification of active nanoparticles, preparation and performance improvement of bone repair scaffolds), and have obtained a series of research results and achievements.

Abstract

Magnesium is an essential element for bone growth. Amorphous calcium phosphate (ACPP) is considered to be the precursor of hydroxyapatite, which has better cell adhesion and degradation ability. It has been reported that Mg-doped ACPP (Mg-nCaP) exhibits better osteogenic and angiogenic activity, but it’s hard to reach a Mg content over 10 wt% and stay amorphous. 

Mg-nCaPs were prepared using a simple and economical ultrasonic method, due to strong complexing ability of pyrophosphate to metal cations, pyrophosphate complexed with Ca2+ and Mg2+. It was found amorphous by XRD. SEM results showed Mg-nCaPs exhibited a colloidal morphology with Mg content of 5%, 10% and 15%. Compared with reactions of monophosphates and polyphosphates, it proved the specific morphology owned to the special complexing of pyrophosphate. BET results proved mesoporous that the pores were mainly composed of interparticle accumulation pores and interlayer pores. The electronegativity and the exposure of multiple binding sites provided Mg-nCaPs controlled release of doxorubicin and bioactive cations, which could achieve stable release of doxorubicin and long-term stable release of Ca2+ and Mg2+ at pH 7.4 and 4.5. Meanwhile, Mg-nCaPs could continuously regulate the physiological alkaline environment, creating superior antibacterial properties. Furthermore, Mg-nCaPs could promote the proliferation, attachment and migration of osteoblasts and endothelial cells, improve ALP activity and mineralization of osteoblasts, enhanced expression of osteogenic and angiogenic genes. Mg-nCaP is a potentially biomaterial for bone repair due to their excellent osteogenic and angiogenic activity.