DivyaPamu

JSS College of Pharmacy, India

Development and Characterization of Repurposed Molecule-Loaded Polycaprolactone Electrospun Nanofibers as a Wound Dressing Biomaterial for Tissue Regeneration

Biography

Abstract

The present study aimed to develop and characterize repurposed molecule-loaded polycaprolactone electrospun nanofibers as a wound dressing biomaterial for tissue regeneration. The electrospun nanofibers were prepared using the electrospinning technique by loading different repurposed drug molecules (atorvastatin, fluvastatin, pravastatin, and rosuvastatin) in polycaprolactone of 80,000 grade at the concentration of 30%. The instrument was set at 0.15-0.5 (?L/min) at 15kV of voltage, and the distance between the needle and the collector mandrel was 15 cm. They characterized SEM, compatibility, thermal analysis, drug release, and invitro MTT assay and migration wound assay in NHDF cells. The polycaprolactone-loaded nanofibers have demonstrated excellent morphology of aligned fibers distribution without any interactions and the nanofibers were found to be compatible and have thermal stability. Among the nanofibers, fluvastatin and pravastatin have shown better release in a controlled manner for 360hrs when compared with atorvastatin and rosuvastatin. In the MTT assay and migration assay, the HDF cells have shown cell proliferation and migration. The fluvastatin and pravastatin have exhibited improved fibroblast cell proliferation and migration in skin tissue regeneration in non-healing wounds. Hence, we concluded that the repurposing of fluvastatin and pravastatin molecules could accelerate the wound healing process by fulfilling the fibroblast deficiency in the tissue and enhancing the further collagen deposition and ECM secretion in the wound region. Further in vivo studies are needed to provide a structured background for commercialization and future clinical purposes.