Feng Cheng

Inner Mongolia Agricultural University, China

Title: Screening of Cholesterol-lowering Lactobacillus and Research on Their Mechanism of Inhibiting Cholesterol Absorption

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Biography

Cheng Feng, female, member of the Communist Party of China, doctoral student at the School of Food Science and Engineering, Inner Mongolia Agricultural University. 2015-2019, undergraduate, major in Cooking and Nutrition Education, Inner Mongolia University of Finance and Economics; 2020 to present, master's and doctoral studies, major in Food Science, Inner Mongolia Agricultural University, Meat Science and Technology Team. Mainly researching the screening and functional characteristics of lactic acid bacteria, the development and application of meat-derived lactic acid bacteria fermentation agents, etc. In 2021, she was approved for the Inner Mongolia Autonomous Region Graduate Research Innovation Project. In 2022-2023, she won the Autonomous Region Academic Scholarship. In 2023, she won the third prize at the college level in the "Internet +" College Students Innovation and Entrepreneurship Competition Project (project leader). In 2024, she published an article in the "Journal of Functional Foods". One patent is being applied for.

Abstract

High cholesterol is one of the important factors causing hyperlipidemia. In this study, a strain of Lactobacillus paracasei JBC1 with in vitro cholesterol-lowering ability and acid and bile salt resistance was isolated and screened from fermented meat products. The effect of JBC1 on inhibiting cholesterol absorption in hypercholesterolemia mice was studied. The mechanism of JBC1's inhibition of cholesterol absorption was analyzed by microbial diversity, liver lipidomics, proteomics and transcriptomics. The results showed that oral administration of JBC1 to hypercholesterolemia mice hindered the weight gain of mice, reduced the total cholesterol (TC) content in the serum and liver of hypercholesterolemia mice, and increased the TC and total bile acid (TBA) content in the feces of hyperlipidemia mice. At the same time, JBC1 can increase the relative expression of ABCG5/G8 in the small intestine of mice and reduce the relative expression of NPC1L1 gene, that is, JBC1 can increase the cholesterol efflux in the small intestine and reduce the cholesterol absorption in the small intestine. JBC1 can increase the intestinal microbial diversity of hypercholesterolemia mice, improve the intestinal microbial structure of hypercholesterolemia mice, and regulate the balance of intestinal flora in hypercholesterolemia mice. The differences in lipidomics, proteomics, and transcriptomics were all enriched in the PPAR signaling pathway, indicating that JBC1 affects cholesterol metabolism by regulating key genes and proteins through PPAR signaling. It mainly reduces cholesterol by promoting cholesterol efflux, and the related genes and proteins are ABCG5 and Cyp4a10. In summary, JBC1 can be used as a functional lactic acid bacterium to intervene in hyperlipidemia.