Shiqi Hao

Inner Mongolia Agricultural University, China

Title: Ability of strains X3-2B and 37X-3 to inhibit xanthine oxidase in vitro and their mitigating effect on hyperuricemia in mice

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Biography

Shiqi Hao is a Ph.D. candidate at Inner Mongolia Agricultural University. His/her main research areas include bioactive peptides, food nutrition, and the analysis of small molecular substances related to nutrition. He/she has published two SCI papers. The research directions of these papers are respectively the study on the alleviating effect of camel milk on non-alcoholic fatty liver disease (NAFLD) in mice, and the improvement of the quality of air-dried meat by vacuum tumbling and marinating.

Abstract

To explore the inhibitory ability of strains X3-2B and 37X-3 on xanthine oxidase (XOD) and the alleviating effect on hyperuricemia (HUA) in mice, the contents of xanthine and uric acid (UA) in the culture environment containing xanthine and XOD with strains X3-2B and 37X-3 were determined. The inhibitory ability of X3-2B and 37X-3 on XOD in vitro and their acid and alkali resistance characteristics were studied. Sixty KM mice were randomly divided into 5 groups: control group (group C), model group (group M), X3-2B group (group X), 37X-3 group (group L) and allopurinol group (group P). Except that group C was fed with control feed, the other groups were fed with high purine feed. After 4 weeks of feeding, mice in group X, group L and group P were respectively intragastrically administered with corresponding doses of strains X3-2B, 37X-3 and allopurinol daily. Through the analysis of physiological and biochemical indexes of mice, renal pathological sections and XOD activity in intestinal tissues, the alleviating effect of strains X3-2B and 37X-3 on HUA in mice was studied. The study showed that strains X3-2B and 37X-3 could effectively inhibit XOD from catalyzing xanthine to produce UA. After 4 weeks of feeding, the contents of UA, BUN and CR in the serum of mice in group X and group L significantly decreased (P<0.05), the renal unit structure was intact, and the XOD activity in intestinal tissues was inhibited (P<0.05). It was concluded that strains X3-2B and 37X-3 could effectively inhibit XOD activity, reduce UA production, and inhibit XOD in the intestine of HUA mice to alleviate HUA.